STAINING TOXOPLASMA GONDII WITH FLUORESCEIN-LABELLED ANTIBODY

Staining Toxoplasma Gondii with Fluorescein-labelled Antibody

A new serologic test for antibodies to Toxoplasma is described, which is based upon inhibition of specific staining with fluorescent antibody. In performing the test, a mixture of the test serum and known fluorescein-labelled antiserum is added to a dried smear of toxoplasms for 1 hour at 37 degrees C. The smear is then rinsed and examined with a fluorescence microscope. Reduction in the bright...

Staining Toxoplasma Gondii with Fluorescein- Labelled Antibody Ii. a New Serologic Test for Antibodies to Toxoplasma Based Itpon Inhibitioi~ O]~ Specific Staining

STAINING TOXOPLASMA GONDII WITH FLUORESCEINo LABELLED ANTIBODY I. Tm~ REACTION IN SMEARS OF PERITONEAL EXUDATE BY MORRIS GOLDMAN, Sc.D

Toxoplasma gondii is a small organism which ordinarily is difficult to identify in tissue sections unless it occurs in clusters known as pseudocysts. A staining method specific for the organism would therefore be of value in the microscopic study of lesions suspected of being caused by this species. Since fluorescein-labelled antibody can be used to stain homologous organisms specifically (refe...

Seroprevalence of Toxoplasma gondii antibody in Vietnamese villagers.

Toxoplasmosis caused by Toxoplasma gondii is a protozoan infection found worldwide. It usually produces non-specific symptoms, but in pregnant women and immunocompomised individuals, it may cause severe and fatal illness. Many serological studies have been done in various parts of the world, but information is lacking for Vietnam. A seroprevalence study of T gondii antibodies in Vietnamese vill...

Toxoplasma gondii

A total of 349 human serum samples were examined for anti-Toxoplasma antibodies by the Murex Single Use Diagnostic System (SUDS) qualitative screening test, indirect hemagglutination assay (IHA), and indirect immunofluorescence assay (IFA). Concordant results with SUDS and IHA were obtained for 91.9% of serum samples; 8.9% were SUDS+ and IHA-; none were SUDSand IHA+. Comparison of the SUDS assa...